Kinetic study on Fermentation of xylose for The Xylitol Production

Efri Mardawati, Andi Trirakhmadi, MTAP Kresnowati, Tjandra Setiadi


Xylitol is a natural sugar that has the sweetness level similar to sucrose, but has lower calorie. It is an important sugar alternate for diabetics people. Reduction of xylose is a normally method to produce the xylitol. It Conducted via chemical hydrogenation of xylose at high pressures and temperatures by reacting pure xylose with hydrogen gas using a metal catalyst. This process requires pure xylose as the raw material. Alternatively, the reduction process can be carried out via fermentation. This process does not require high purity of xylose as the raw material, and thus the oil palm empty fruit bunch (EFB) hydrolysate, without any prior pretreatment, can be used.  In order to scale up the xylitol production via fermentation, kinetic study of xylitol fermentation including growth and xylitol formation kinetic using the synthetic xylose as substrate will be required.  Data used in the kinetic model development were obtained from series of batch fermentations of Debaryomycess hansenii ITB CCR85 varying the initial xylose and glucose concentrations. Yeast growth could be sufficiently modeled using the Monod kinetics, whereas xylitol production could be reasonably well modelled by Luedeking Piret kinetics.

Full Text:



Musatto, S.I. Silva, C. and Roberto, I.C. Fermentation performance of Candida guilliermondii for xylitol production on single and mixed substrate media. Appl Microbiol Biotechnol 72,2006, 681–686

Oh, D.K. and Kim, S.Y. Increase of xylitol yield by feeding xylose and glucose in Candida tropicalis. Appl Microbiol Biotechnol 50, 1998, 419-425.

Parajo, J.C. Dominguez, H. and Dominguez, J.M. Biotechnological production of xylitol. Part 1: Interest of Xylitol and Fundamentals of Its biosynthesis, Bioresource Technology 65, 1998, 191-201

Converti, A. Perego, P. Sordi, A. and Torre, P. Effect of Starting Xylose Concentration on the Microaerobic Metabolism of Debaryomyces hansenii. Applied Biochemistry and Biotechnology 101, 2002, 15-29

Dominguez, J. Gong, C.S., and Tsao. Production of Xylitol from D-Xylose by Debaryomyces hansenii. Applied Biochemistry and Biotechnology 63-65, 1997, 117-127

Mardawati, E, Wira, D.W., Kresnowati., MTAP, Purwadi, R., and Setiadi, T. Microbial Production of Xylitol From Oil Palm Empty Fruit Bunches Hydrolysate : The Effect of Glucose Concentration, Journal of Jpn. Inst. Energy 94 (8), 2015, 769-774

Sampaio, FC. Torre, P. Passos, FML. Moraes, CA. Perego, P. and Converti, A. Influence of Inhibitory Compounds and Minor Sugars on Xylitol Production by Debaryomyces hansenii, Applied Biochemistry and Biotechnology 136, 2003, 165-181

Parajo, J.C. Dominguez, H and Dominguez, H.M. Xylitol From Wood : Study of Some Strategies. Food Chemistry 57 (4), 1996, 531-536

Silva, S.S. Felipe,, M. and Mancilha, I.M. Factors that Affect the Biosynthesis of Xylitol by Xylosa Fermenting Yeasts- A Review. Applied Biochemistry and Biotechnology 70-72, 1998, 331

Shuler, M.L. and F. Kargi. Biopprocess Engineering Basic Concepts. Prentice-Hall, Inc. New Jersey, 1992

Tochampa, W. Sirisansaneeyakul, S. Vanichsriratana, W. Srinophakun, P. Bakker, HH., and Chisti, Y., A. model of xylitol production by the yeast Candida mogii, Bioprocess Biosyst Eng, 28(3), 2005, 175-83.


  • There are currently no refbacks.