Review Article: Effect of Co-Expression Chaperones on the Expression of Intracellular Recombinant Proteins in Escherichia coli

Abstract

Escherichia coli is widely used as a host for expressing recombinant proteins due to its well-studied genetics, fast growth, relatively low production costs, and high rate of protein expression. However, despite the high rate of protein expression, the availability of chaperone proteins was often insufficient, resulting in the formation of inclusion bodies due to errors in protein folding. These inclusion bodies can cause the protein to become inactive, and proper protein folding is crucial for maintaining the structure and function of proteins in living organisms. To overcome this limitation, chaperones have been developed as a strategy to help prevent protein folding errors and increase the recovery of soluble protein. In this review, we summarize several experiments related to co-expressing chaperones to enhance the expression of recombinant proteins in E. coli.

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Volume

11

Issue

cna/vol11/iss2

Authors

Diandra Firdiani Utami, Padjadjaran UniversityFollow
Mamlikatu Ilmi Azizah
Sriwidodo Sriwidodo
Ryan Adibagus Haryanto
Riyona Desvy Pratiwi
Iman Permana Maksum

Recommended Citation

Utami, Diandra Firdiani; Azizah, Mamlikatu Ilmi; Sriwidodo, Sriwidodo; Haryanto, Ryan Adibagus; Pratiwi, Riyona Desvy; and Maksum, Iman Permana (2023) "Review Article: Effect of Co-Expression Chaperones on the Expression of Intracellular Recombinant Proteins in Escherichia coli," Chimica et Natura Acta: Vol. 11: Iss. 2, Article 6.
DOI: https://doi.org/10.24198/cna.v11.n2.46480
Available at: https://journal.unpad.ac.id/cna/vol11/iss2/6