Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP

Authors

Suci Rahayu, Badan Riset dan Inovasi NasionalFollow
Darmawan Saptadi, Universitas BrawijayaFollow
Chotimatul Azmi, Badan Riset dan Inovasi NasionalFollow
Kusumawaty Kusumanegara, Badan Riset dan Inovasi NasionalFollow

Abstract

Centella's need for industrial raw materials is high as a medicinal plant. These needs can be met through rapid multiplication using tissue culture techniques. In this study, induction and proliferation for a callus of centella cv. Castina 3 was conducted in the MS basal medium plus 4 mgL-1 Dicamba withand enriched with 7 concentrations of BAP (0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.1 mgL-1). Two kinds of explant were used, i.e., leaf and petiole. The results revealed that the addition of BAP in MS plus Dicamba medim stimulated better and produced a higher callus growth rate, both from leaf and petiole explants, than that media with Dicamba alone. Furthermore, 4 mgL-1 Dicamba + 1.1 mgL-1 BAP had a friable callus in the induction phase and a friable-compact callus in the proliferation phase. From this finding, it can be considered to use a combination of 4 mgL-1 Dicamba with 1.1 mgL-1 BAP in callus induction and proliferation for Centella rapid multiplication.

Recommended Citation

Rahayu, Suci; Saptadi, Darmawan; Azmi, Chotimatul; and Kusumanegara, Kusumawaty (2023) "Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP," Jurnal Kultivasi: Vol. 22: No. 3, Article 13.
Available at: https://journal.unpad.ac.id/kultivasi/vol22/iss3/13