Assessment of Pletekan Leaf Extract (Ruellia tuberosa L.) as an Antibacterial Feed Additive for Broiler Chickens (Gallus gallus domesticus L.) Infected with Salmonella sp.

Authors

• Nuria -. Istiqomah, Master of Forensic Sciences, Postgraduate School, Universitas Airlangga, 4-6 Airlangga Rd, Surabaya, 60286, IndonesiaFollow
• Poncojari Wahyono, Department of Biology Education, Faculty of Teacher Training and Education, Muhammadiyah University of MalangFollow
• Rury Eryna Putri, Departement of Forensic Sciences, Postgraduate School, Universitas Airlangga, 4-6 Airlangga Rd, Surabaya, 60286, IndonesiaFollow
• Abdulkadir Rahardjanto, Department of Biology Education, Faculty of Teacher Training and Education, Muhammadiyah University of MalangFollow

Author ORCID Identifier

Nuria Istiqomah, https://orcid.org/0009-0007-0901-2576

Poncojari Wahyono, https://orcid.org/0009-0003-7965-1913

Rury Eryna Putri, https://orcid.org/0009-0005-9924-1852

Abdulkadir Rahardjanto, https://orcid.org/0009-0009-3809-0738

Abstract

The use of synthetic antibiotics as antibiotic growth promoters (AGPs) in broiler chickens raises concerns over antibiotic residues and bacterial resistance, necessitating safer natural alternatives. Ruellia tuberosa L. contains various secondary metabolites with potential antibacterial properties; however, its in vivo efficacy against Salmonella sp. remains poorly documented. This study aimed to analyze the phytochemical composition of R. tuberosa leaf extract and evaluate its antibacterial activity against Salmonella sp. in broiler chickens. A Completely Randomized Design (CRD) was employed with eight treatments: a positive control (tetracycline), a negative control (Tween-80), and six extract concentrations (125, 250, 375, 500, 625, and 1000 ppm), each replicated four times. Antibacterial activity was assessed by counting Salmonella sp. in feces using the MPN method and analyzed with the Kruskal-Wallis test (α = 5%). Phytochemical screening revealed the presence of tannins and alkaloids in the extract. The highest mean Salmonella sp. count was at 125 ppm (1,1775 MPN/g) and the lowest in the negative control (0.3 MPN/g). Statistical analysis showed no significant effect of the extract (p = 0.814). It was concluded that R. tuberosa leaf extract at 125-1000 ppm was not effective as an antibacterial agent against Salmonella sp. in broiler chickens in vivo.

Recommended Citation

Istiqomah, Nuria -.; Wahyono, Poncojari; Putri, Rury Eryna; and Rahardjanto, Abdulkadir () "Assessment of Pletekan Leaf Extract (Ruellia tuberosa L.) as an Antibacterial Feed Additive for Broiler Chickens (Gallus gallus domesticus L.) Infected with Salmonella sp.," Jurnal Ilmu Ternak Universitas Padjadjaran: Vol. 26: No. 3, Article 9.
DOI: https://doi.org/10.24198/jit.v26i3.1486
Available at: https://journal.unpad.ac.id/jitunpad/vol26/iss3/9